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KMID : 1149220190270010007
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Korean Journal of Oriental Medical Prescription
2019 Volume.27 No. 1 p.7 ~ p.16
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Anti-inflammatory effect of Hemistepta lyrata Bunge (Bunge) on LPS- induced inflammation in RAW 264.7 cells
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Kim Jae-Kwang
Park Su-Young
Choi Hwa-Young
Jang Mi-Hee
Jung Dae-Hwa
Kim Sang-Chan
Cho Il-Je
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Abstract
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Objectives : Hemistepta lyrata Bunge (Bunge) has been used for treating wound, hemorrhage, fever in Korean traditional medicine. Present study investigated anti-inflammatory effect of H. lyrata chloroform extract (HLE) and its molecular mechanism involved.
Methods : To assess anti-inflammatory effect of HLE, production of nitric oxide (NO) and expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines were measured on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Moreover, nuclear factor-κB (NF-κB) signaling pathway was examined to elucidate its molecular mechanism.
Results : Pretreatment of HLE inhibited NO production in a concentration dependent manner. HLE also decreased expression of iNOS and COX-2, and alleviated expressions of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. Moreover, HLE pretreatment inhibited phosphorylation of inhibitory-κBα and p65.
Conclusions : These results suggest that HLE exhibits anti-inflammatory effect via inhibition of NF-κB.
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KEYWORD
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Hemistepta lyrata Bunge (Bunge), RAW 264.7 cells, Inflammation, Nuclear factor-κB (NF-κB)
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